Set:
Yesterday, we talked about four types of technology that are used to create a DNA fingerprint. Does anyone remember one of the four types we discussed?
[PCR, RFLPs, gel electrophoresis, and probing]
Today, we are going to review those technologies, as well as transcription, translation, and gene mutations. And to do that, we’re going to play a simple game; except, this time there are no teams. So, you’re playing for yourself, in order to win one of these fabulous prizes. And, here’s the catch, we will only have three winners today (1st, 2nd, & 3rd place in points).
Here are the rules:
1. We are going to start the game off with Shanequia having the opportunity to answer the first question.
2. If you do not answer immediately, you will be given ten seconds to answer. If you answer incorrectly, the next person gets the chance to answer a different question.
3. Incorrectly answered questions will be put back in the stack, so they may be called again.
4. You can not receive or give help to another player, or you lose your turn.
5. Each correctly answered question is worth one point.
(20 minutes)
Procedure:
1. Place the open-book quizzes on the students’ desks before the bell rings (see above Do Now).
2. Begin teacher-led discussion, reviewing transcription, translation, gene mutations and DNA technology through the flash-card game:
i. Explain the rules of the game first (see above Set)
3. Play videos on PCR, gel electrophoresis & DNA rap
i. Have students get out their DNA technology review books from yesterday.
ii. Instruct students to add DNA Polymerase as the catalyst for PCR
iii. Instruct students to add agarose gel under gel electrophoresis tab
(10 minutes)
4. Closure
Closure:
We spent today’s lesson reviewing transcription, translation, DNA mutations and DNA technologies. After your test tomorrow, we are going to uncover a cloning process that is conducted at the molecular level in laboratories. It’s pretty interesting because it uses bacterial cells to replicate the desired DNA. Think about the HIV virus for just a moment. When the HIV virus attacks cells, it changes the DNA so that it stops replicating the cell’s normal DNA and replicates more HIV cells. With recombinant DNA technology, the same process is occurring except it is in a laboratory, and the experiment is controlled to replicate more of the desired DNA.
(5 minutes)
Assessment/ Evaluation:
Objective(s):
Utilizing their knowledge of transcription, translation, gene mutation and DNA technology to review for test (DOK 2 Biology, 5.c).
Informal: The teacher will listen to the students’ responses during the flash-card game (M), prompting for definitions and concepts of transcription, translation, gene mutations and DNA technology (C).
Formal: The teacher will retrieve the students’ open book quizzes, covering the
aforementioned areas, check for accuracy and completion of the material (C), and will
record a “complete” grade in the grade book (D).
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